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One of these was Henri Becquerel, who investigated the relationship between phosphorescence and the blackening of photographic plates. When Becquerel discovered that, with no external source of energy, the uranium generated rays which could blacken the photographic plate, radioactivity was discovered. Marie Skłodowska-Curie and her husband Pierre Curie isolated two new radioactive elements from uranium ore. They then attempted to separate these radioactive fractions further, to isolate a smaller fraction with a higher specific activity .
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Thus, these methods investigate the local structure in matter, mainly condensed matter in condensed matter physics and solid state chemistry. The isotope emits very soft beta radiation, and can be detected by scintillation counting. For biochemical and physiological experiments and medical methods, a number of specific isotopes have important applications. Within living things, isotopic labels can be used to probe how the complex web of reactions which makes up the metabolism of an organism converts one substance to another. For instance a green plant uses light energy to convert water and carbon dioxide into glucose by photosynthesis. If the oxygen in the water is labeled, then the label appears in the oxygen gas formed by the plant and not in the glucose formed in the chloroplasts within the plant cells.
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